AP51071PU-N

antibody from Acris Antibodies GmbH

Targeting: CREB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP51071PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP51071PU-N, RRID:AB_11151753

Product name

anti CREB1 (Center)

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 112-140 amino acids from the Central region of human CREB1

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.4 ml

Concentration

lot specific

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

CREB1 Antibody (Center) (Cat. #AP51071PU-N) western blot analysis in mouse cerebellum tissue lysates (35µg/lane).This demonstrates the CREB1 antibody detected the CREB1 protein (arrow).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of CREB1 (arrow) using rabbit polyclonal CREB1 Antibody (Center) (Cat. #AP51071PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CREB1 gene.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of CREB1 Antibody (Center)(Cat#AP51071PU-N) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

CREB1 Antibody (Center) (Cat. #AP51071PU-N) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.