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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- BM4007R - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#BM4007R, RRID:AB_11218379
- Product name
- anti Macrophage F4/80 antigen
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- BM8
- Vial size
- 100 Tests
- Concentration
- 0.1 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Mouse anti-Macrophage F4/80 antigen antibody Cat.-No BM4007F (5 µg/ml) on Raw 204.4 cells. Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- F4/80 expression on macrophages in mouse liver. Staining of frozen tissue section with purified antibody BM8
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Mouse anti-Macrophage F4/80 antigen antibody Cat.-No BM4007F on thioglycollate elicited mouse peritoneal macrophages. Purple: BM4007F; Green: Isotype control SM15F; Percentages reflect % positive after subtraction of negative control, using M1 marker.
