BM4007RT

antibody from Acris Antibodies GmbH

Targeting: ADGRE1 EMR1, TM7LN3

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

BM4007RT

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#BM4007RT, RRID:AB_11220168

Product name

anti Macrophage F4/80 antigen

Antibody type

Monoclonal

Antigen

Other

Reactivity

Mouse

Host

Rat

Isotype

IgG

Antibody clone number

BM8

Vial size

25 Tests

Concentration

0.1 mg/ml

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Mouse anti-Macrophage F4/80 antigen antibody Cat.-No BM4007F (5 µg/ml) on Raw 204.4 cells. Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

F4/80 expression on macrophages in mouse liver. Staining of frozen tissue section with purified antibody BM8

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Mouse anti-Macrophage F4/80 antigen antibody Cat.-No BM4007F on thioglycollate elicited mouse peritoneal macrophages. Purple: BM4007F; Green: Isotype control SM15F; Percentages reflect % positive after subtraction of negative control, using M1 marker.