R1189

antibody from Acris Antibodies GmbH

Targeting: CREB1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

R1189

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1189, RRID:AB_978960

Product name

anti CREB1 pSer133

Antibody type

Polyclonal

Antigen

CREB phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

0.1 ml

Concentration

0.064 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Anti-CREB pS133 was used to detect phosphorylated CREB by immunoblot. Recombinant His- tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. Immunoblot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Pan reactive CREB (R1188) reacts equally with both nonphosphorylated and phosphorylated CREB (not shown). Detection occurs using a 1:1,000 dilution of antibody followed by 1:5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results. Data contributed by Boss, J., Emory University School of Medicine, personal communication.