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LearnR1472P
antibody from Acris Antibodies GmbH
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- R1472P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1472P, RRID:AB_1005407
- Product name
- anti mTOR / FRAP1 pSer2448
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to amino acids 2440-2457 of Human mTOR
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 1.88 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoblotting. R1472P mTOR antibody (pSer2448) is shown to detect a 250kDa band (indicated) corresponding to phosphorylated Human mTOR present in a 293Twhole cell lysates. Cells were serumstarved for 24 hours prior to harvest.~20μg of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in Lane 1, whereas cells in Lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1/2000 dilution of HRPGoat anti-Rabbit IgG for visualization.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: R1472P pSer2448 mTOR antibody staining of Formalin-Fixed, Paraffin Embedded Sections. This antibody was used at 5 μg/ml to detect signal in a variety of tissues including multi-Human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney. The image shows localization of the antibody as the precipitated Red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, Seattle, WA.
