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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- BM4007 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#BM4007, RRID:AB_1618214
- Product name
- anti Macrophage F4/80 antigen
- Antibody type
- Monoclonal
- Antigen
- Cultured macrophages.The F4/80 antigen is a 125 kD extracellular membrane protein sensitive to 2-Mercaptoethanol.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- BM8
- Vial size
- 0.2 mg
- Concentration
- 0.4 mg/ml (after reconstitution)
Submitted references Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody.
Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis.
Differential regulation of the nature and functions of dendritic cells and macrophages by cathepsin E.
Reduced in vivo aortic uptake of radiolabeled oxidation-specific antibodies reflects changes in plaque composition consistent with plaque stabilization.
Stangl S, Gehrmann M, Riegger J, Kuhs K, Riederer I, Sievert W, Hube K, Mocikat R, Dressel R, Kremmer E, Pockley AG, Friedrich L, Vigh L, Skerra A, Multhoff G
Proceedings of the National Academy of Sciences of the United States of America 2011 Jan 11;108(2):733-8
Proceedings of the National Academy of Sciences of the United States of America 2011 Jan 11;108(2):733-8
Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis.
Kimura H, Miyashita H, Suzuki Y, Kobayashi M, Watanabe K, Sonoda H, Ohta H, Fujiwara T, Shimosegawa T, Sato Y
Blood 2009 May 7;113(19):4810-8
Blood 2009 May 7;113(19):4810-8
Differential regulation of the nature and functions of dendritic cells and macrophages by cathepsin E.
Kakehashi H, Nishioku T, Tsukuba T, Kadowaki T, Nakamura S, Yamamoto K
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 1;179(9):5728-37
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 1;179(9):5728-37
Reduced in vivo aortic uptake of radiolabeled oxidation-specific antibodies reflects changes in plaque composition consistent with plaque stabilization.
Torzewski M, Shaw PX, Han KR, Shortal B, Lackner KJ, Witztum JL, Palinski W, Tsimikas S
Arteriosclerosis, thrombosis, and vascular biology 2004 Dec;24(12):2307-12
Arteriosclerosis, thrombosis, and vascular biology 2004 Dec;24(12):2307-12
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Mouse anti-Macrophage F4/80 antigen antibody Cat.-No BM4007F (5 µg/ml) on Raw 204.4 cells. Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- BM4007 F4/80 Antigen antibody staining of Mouse Liver Frozen Section.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- BM4007 F4/80 antibody staining of Mouse Spleen Frozen.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- BM4007 F4/80 antibody staining of Mouse Liver Section.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Mouse anti-Macrophage F4/80 antigen antibody Cat.-No BM4007F on thioglycollate elicited mouse peritoneal macrophages. Purple: BM4007F; Green: Isotype control SM15F; Percentages reflect % positive after subtraction of negative control, using M1 marker.
