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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP08619PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP08619PU-N, RRID:AB_1975496
- Product name
- anti CDK1 pTyr15
- Antibody type
- Polyclonal
- Antigen
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr15 of rat CDC2.
- Reactivity
- Human, Mouse, Rat, Xenopus, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Figure 1. Western blot of human T47D cells showing specific immunolabeling of the ~34k cdc2 phosphorylated at Tyr15 (Control). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). The phosphospecificity of this labeling is also shown in the third and fourth lanes (lambda-phosphatase). These blots are identical to the control except that they were incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr15 cdc2. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
