AP09242PU-N

antibody from Acris Antibodies GmbH

Targeting: PRKDC DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, p350, p460, XRCC7

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09242PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09242PU-N, RRID:AB_2035417

Product name

anti DNA-PKcs / PRKDC / XRCC7 pThr2609

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids surrounding Thr 2609 of Human DNA PKcs

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.93 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using AP09242PU-N DNAPKcs antibody shows detection of a 460 kDa band corresponding to Human DNAPKcs in various preparations. Lanes 1-5 are nuclear extract whereas Lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNAPKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 μg of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1/1,000 dilution of AP09242PU-N DNAPKcs antibody. Detection occurred using a 1/5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at RT. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h) Lane 3: Fus1 DNAPK inhibitor + IR Lane 4: MO59J (DNAPK-) untreated Lane 5: MO59J IR, Lane 6: Fus1 untreated Lane 7: Fus1 IR (20Gy, 4h) Lane 8: Fus1 DNAPK inhibitor + IR Lane 9: MO59J untreated Lane 10: MO59J IR