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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Western blot [3]
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- Product number
- NB100-323 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-323, RRID:AB_10003234
- Product name
- Rabbit Polyclonal ATR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references A type I IFN-dependent DNA damage response regulates the genetic program and inflammasome activation in macrophages.
Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation.
Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis.
Gallic acid causes inactivating phosphorylation of cdc25A/cdc25C-cdc2 via ATM-Chk2 activation, leading to cell cycle arrest, and induces apoptosis in human prostate carcinoma DU145 cells.
Morales AJ, Carrero JA, Hung PJ, Tubbs AT, Andrews JM, Edelson BT, Calderon B, Innes CL, Paules RS, Payton JE, Sleckman BP
eLife 2017 Mar 31;6
eLife 2017 Mar 31;6
Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation.
Min W, Bruhn C, Grigaravicius P, Zhou ZW, Li F, Krüger A, Siddeek B, Greulich KO, Popp O, Meisezahl C, Calkhoven CF, Bürkle A, Xu X, Wang ZQ
Nature communications 2013;4:2993
Nature communications 2013;4:2993
Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
Boichuk S, Hu L, Hein J, Gjoerup OV
Journal of virology 2010 Aug;84(16):8007-20
Journal of virology 2010 Aug;84(16):8007-20
Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis.
Shao L, Fujii H, Colmegna I, Oishi H, Goronzy JJ, Weyand CM
The Journal of experimental medicine 2009 Jun 8;206(6):1435-49
The Journal of experimental medicine 2009 Jun 8;206(6):1435-49
Gallic acid causes inactivating phosphorylation of cdc25A/cdc25C-cdc2 via ATM-Chk2 activation, leading to cell cycle arrest, and induces apoptosis in human prostate carcinoma DU145 cells.
Agarwal C, Tyagi A, Agarwal R
Molecular cancer therapeutics 2006 Dec;5(12):3294-302
Molecular cancer therapeutics 2006 Dec;5(12):3294-302
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATR Antibody [NB100-323] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ATR antibody NB100-323 lot 3 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ATR was also immunoprecipitated by a previous lot (lot 2) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATR Antibody [NB100-323] - H1299 (human lung cancer cells) and HCC 44 (human liver cancer cells). Antibody at 1:500. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
