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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP1-71835 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-71835, RRID:AB_11003340
- Product name
- Rabbit Polyclonal LRRFIP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LRRFIP1 Antibody [NBP1-71835] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (50 ug) cells. Affinity purified rabbit anti-LRRFIP1 antibody used for WB at 0.04 ug/mL (A) and 0.4 ug/mL (B) and used for IP at 6 ug/mg lysate. LRRFIP1 was also immunoprecipitated by rabbit anti-LRRFIP1 antibodies NBP1-71834 and NBP1-71836, which recognize other epitopes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: LRRFIP1 Antibody [NBP1-71835] - LRRFIP1 IP in B-cell whole cell lysate. 2 ug antibody : 500 ug whole cell lysate. Same antibody used at 1:2000 for Western blot. Image submitted by a verified customer review.
