Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-87035 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87035, RRID:AB_11010816
- Product name
- Rabbit Polyclonal NSF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human NSF antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Spatiotemporal changes in NSF expression of DRG neurons in a rat model of spinal nerve ligation.
Li X, Zou Y, Luo H, Weng Y, Guo Q, Huang C
Journal of molecular neuroscience : MN 2014 Aug;53(4):645-53
Journal of molecular neuroscience : MN 2014 Aug;53(4):645-53
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NSF Antibody [NBP1-87035] - Analysis in human cell line A-549.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NSF Antibody [NBP1-87035] - Analysis in control (vector only transfected HEK293T lysate) and NSF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human testis shows cytoplasmic positivity in cells of seminiferus ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human cerebral cortex shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Analysis in human cerebral cortex and liver tissues. Corresponding NSF RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human cerebral cortex shows strong positivity in neuropil.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human liver shows no positivity in hepatocytes as expected.