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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB38981 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Calreticulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Calreticulin in ELISA. Detects human, mouse and rat Calreticulin in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Enhanced validation
- Submitted by
- Novus Biologicals (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Calreticulin Monoclonal Antibody (Catalog # MAB38981) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Calreticulin by Western Blot. Detection of Human, Mouse, and Rat Calreticulin by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Calreticulin in Human Prostate. Calreticulin in Human Prostate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Calreticulin in HeLa Human Cell Line by Flow Cytometry. Detection of Calreticulin in HeLa Human Cell Line by Flow Cytometry.
