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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- F40308 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- UCHL1 Antibody
- Antibody type
- Monoclonal
- Antigen
- This UCHL1 antibody was produced from a mouse immunized with UCHL1 recombinant protein.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 346CT2.2.1
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the UCHL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from 1) NCI-H460, 2) U266, 3) mouse Neuro-2a, rat 4) PC-12 and 5) C6 cell line and 6) mouse brain tissue using UCHL1 antibody at 1:1000. Predicted molecular weight ~25 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of A549 cells stained with UCHL1 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney using UCHL1 at 1:25 dilution.
