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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-19787 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Follistatin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For Western Blot, this antibody has non-specific bands at 15 kDa and 75 kDa.
- Concentration
- 1 mg/mL
Submitted references Skeletal Muscle Anti-Atrophic Effects of Leucine Involve Myostatin Inhibition.
Secretome profiling of PC3/nKR cells, a novel highly migrating prostate cancer subline derived from PC3 cells.
A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood.
Cruz A, Ferian A, Alves PKN, Silva WJ, Bento MR, Gasch A, Labeit S, Moriscot AS
DNA and cell biology 2020 Nov 2;
DNA and cell biology 2020 Nov 2;
Secretome profiling of PC3/nKR cells, a novel highly migrating prostate cancer subline derived from PC3 cells.
Jeon JM, Kwon OK, Na AY, Sung EJ, Cho IJ, Kim M, Yea SS, Chun SY, Lee JH, Ha YS, Kwon TG, Lee S
PloS one 2019;14(8):e0220807
PloS one 2019;14(8):e0220807
A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood.
Rebo J, Mehdipour M, Gathwala R, Causey K, Liu Y, Conboy MJ, Conboy IM
Nature communications 2016 Nov 22;7:13363
Nature communications 2016 Nov 22;7:13363
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Raji Whole Cell Lysate using Product # PA5-19787, Follistatin primary antibody at a dilution of 1 µg/mL (lane 1). Staining of MOLT4 Whole Cell Lysate at a dilution of 1 µg/mL (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 3 Validation of quantitative proteomics result using immunoblots. (A) Western blotting analysis of proteins with increased (Crk, SerpinB3, Cystatin-M), decreased (ULBP2), and unchanged secretion (Follistatin) in conditioned media (CM) and cell extract (CE) samples from PC3/nKR cells as compared with those from PC3 cells. (B) Densitometry results of Crk, SerpinB3, Cystatin-M, ULBP2 and Follistatin.
