39301

antibody from Active Motif

Targeting: RUNX3 AML2, CBFA3, PEBP2A3

Western blot (Recommended by provider)

Immunoelectron microscopy (Recommended by provider)

Antibody Data

Product number

39301

Provider

Active Motif

Proper citation

Active Motif Cat#39301, RRID:AB_2793222

Product name

AML-2/Runx3 antibody (pAb)

Antibody type

Polyclonal

Description

This AML-2 antibody (RUNX3) has been validated for use in Western blot (1:500 - 1:1,000 dilution) and electrophoretic mobility shift assays (EMSA). We recommend adding 4-6 µl of antibody to the reaction mixture when performing supershift experiments. We also recommend the use of Tris-glycine electrophoresis buffer. For Western blot experiments, a 44 kDa band should be detected. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Reactivity

Human

Host

Rabbit

Vial size

100 µl

Storage

Antibodies in solution can be stored at -80C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.