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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB797 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IGFBP-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IGFBP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IGFBP-1, -2, -3, -4, -5, -6, recombinant mouse IGFBP-1, -3, -5, or -6 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
CAA57270- Isotype
- IgG
- Antibody clone number
- 150101
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Patched1 inhibits epidermal progenitor cell expansion and basal cell carcinoma formation by limiting Igfbp2 activity.
Villani RM, Adolphe C, Palmer J, Waters MJ, Wainwright BJ
Cancer prevention research (Philadelphia, Pa.) 2010 Oct;3(10):1222-34
Cancer prevention research (Philadelphia, Pa.) 2010 Oct;3(10):1222-34
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IGFBP-2 Inhibition of IGF-II-dependent Cell Proliferation and Neutralization by Mouse IGFBP-2 Antibody. Recombinant Mouse IGFBP-2 (Catalog # 797-B2) inhibits Recombinant Mouse IGF-II (Catalog # 792-MG) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IGF-II (30 ng/mL) activity elicited by Recombinant Mouse IGFBP-2 (0.75 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IGFBP-2 Monoclonal Antibody (Catalog # MAB797). The ND50 is typically 5-20 µg/mL.
