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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-29409 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-HRI Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-29409 targets EIF2AK1 in IF, IHC (P), and WB applications and shows reactivity with Human samples. The PA5-29409 immunogen is recombinant fragment corresponding to a region within amino acids 304 and 601 of Human EIF2AK1. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF2AK1 using A) 30 µg HeLa whole cell lysate and B) 30 µg A375 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an EIF2AK1 polyclonal antibody (Product # PA5-29409) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of EIF2AK1 in paraffin-embedded H661 xenograft using an EIF2AK1 polyclonal antibody (Product # PA5-29409) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EIF2AK1 in paraformaldehyde-fixed A431 cells using an EIF2AK1 polyclonal antibody (Product # PA5-29409) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HRI was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of polyclonal HRI antibody (Product # PA5-29409) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.
