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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX114455 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114455, RRID:AB_11167162
- Product name
- TFIIF antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Resources for the Comprehensive Discovery of Functional RNA Elements.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7.5% SDS PAGE GTX114455 diluted at 1:3000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX114455 diluted at 1:3000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TFIIF antibody immunoprecipitates TFIIF protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 £gg whole cell lysate/extract of TFIIF protein expressing 293T cells B : Control with 2.5 £gg of pre-immune rabbit IgG C : Immunoprecipitation of TFIIF by 2.5 £gg of TFIIF antibody (GTX114455) 7.5% SDS-PAGE The immunoprecipitated TFIIF protein was detected by TFIIF antibody (GTX114455) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 £gg of either control rabbit IgG or anti-TFIIF antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
