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- References [4]
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- NBP2-16957 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal IL-6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Chemotherapy-Induced Ca2+ Release Stimulates Breast Cancer Stem Cell Enrichment.
A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8.
Walnut Phenolic Extract and Its Bioactive Compounds Suppress Colon Cancer Cell Growth by Regulating Colon Cancer Stemness.
Hypoxia-inducible factors are required for chemotherapy resistance of breast cancer stem cells.
Lu H, Chen I, Shimoda LA, Park Y, Zhang C, Tran L, Zhang H, Semenza GL
Cell reports 2017 Feb 21;18(8):1946-1957
Cell reports 2017 Feb 21;18(8):1946-1957
A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8.
Ahlstrand T, Tuominen H, Beklen A, Torittu A, Oscarsson J, Sormunen R, Pöllänen MT, Permi P, Ihalin R
Virulence 2017 Feb 17;8(2):115-134
Virulence 2017 Feb 17;8(2):115-134
Walnut Phenolic Extract and Its Bioactive Compounds Suppress Colon Cancer Cell Growth by Regulating Colon Cancer Stemness.
Lee J, Kim YS, Lee J, Heo SC, Lee KL, Choi SW, Kim Y
Nutrients 2016 Jul 21;8(7)
Nutrients 2016 Jul 21;8(7)
Hypoxia-inducible factors are required for chemotherapy resistance of breast cancer stem cells.
Samanta D, Gilkes DM, Chaturvedi P, Xiang L, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 16;111(50):E5429-38
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 16;111(50):E5429-38
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-6 Antibody [NBP2-16957] - IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-6 Antibody [NBP2-16957] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-6 Antibody [NBP2-16957] - IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody diluted at 1:2250.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-16957] - Analysis of paraffin-embedded Breast CA, using antibody at 1:250 dilution.
