AHC0762

antibody from Invitrogen Antibodies

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

AHC0762

Provider

Invitrogen Antibodies

Product name

IL-6 Monoclonal Antibody (8H12)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

8H12

Vial size

250 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

Inflammatory conversion of quiescent osteoblasts by metastatic breast cancer cells through pERK1/2 aggravates cancer-induced bone destruction.

Back J, Nguyen MN, Li L, Lee S, Lee I, Chen F, Gillinov L, Chung YH, Alder KD, Kwon HK, Yu KE, Dussik CM, Hao Z, Flores MJ, Kim Y, Ibe IK, Munger AM, Seo SW, Lee FY
Bone research 2021 Sep 29;9(1):43

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Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.

Buken C, Sahana J, Corydon TJ, Melnik D, Bauer J, Wehland M, Krüger M, Balk S, Abuagela N, Infanger M, Grimm D
Scientific reports 2019 Aug 15;9(1):11882

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IL-6 showing staining in the cytoplasm of paraffin-embedded human spleen tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a IL-6 monoclonal antibody (Product # AHC0762) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.