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LearnNB600-1131
antibody from Novus Biologicals
Targeting: IL6
BSF2, HGF, HSF, IFNB2, IL-6
Western blot
ELISA Immunocytochemistry Immunoprecipitation Immunohistochemistry Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [26]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB600-1131 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-1131, RRID:AB_10001997
- Product name
- Rabbit Polyclonal IL-6 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. This antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Vial size
- 0.2 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references TonEBP-deficiency accelerates intervertebral disc degeneration underscored by matrix remodeling, cytoskeletal rearrangements, and changes in proinflammatory gene expression.
Analysis of inflammatory cytokine expression in the urinary tract of BALB/c mice infected with Proteus (P.) mirabilis and enteroaggregative Escherichia (E.) coli (EAEC) strains.
Acute changes of pro-inflammatory markers and corticosterone in experimental subarachnoid haemorrhage: A prerequisite for severity assessment.
Silencing of A20 Aggravates Neuronal Death and Inflammation After Traumatic Brain Injury: A Potential Trigger of Necroptosis.
p16(Ink4a) deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice.
Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense.
Erlotinib overcomes paclitaxel-resistant cancer stem cells by blocking the EGFR-CREB/GRβ-IL-6 axis in MUC1-positive cervical cancer.
Interleukin-12 and -23 blockade mitigates elastase-induced abdominal aortic aneurysm.
Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon.
RNA inhibitors of nuclear proteins responsible for multiple organ dysfunction syndrome.
Cell-type-specific role of lamin-B1 in thymus development and its inflammation-driven reduction in thymus aging.
BMP-2/7 heterodimer strongly induces bone regeneration in the absence of increased soft tissue inflammation.
Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α.
Transgenic mice overexpressing human TNF-α experience early onset spontaneous intervertebral disc herniation in the absence of overt degeneration.
Macropinocytosis of Nab-paclitaxel Drives Macrophage Activation in Pancreatic Cancer.
Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection.
Targeted CFTR gene disruption with zinc-finger nucleases in human intestinal epithelial cells induces oxidative stress and inflammation.
AAV8-mediated expression of N-acetylglucosamine-1-phosphate transferase attenuates bone loss in a mouse model of mucolipidosis II.
Porphyromonas gingivalis GroEL induces osteoclastogenesis of periodontal ligament cells and enhances alveolar bone resorption in rats.
Autophagy modulates SNCA/α-synuclein release, thereby generating a hostile microenvironment.
Adipocyte lipolysis-stimulated interleukin-6 production requires sphingosine kinase 1 activity.
Antibody-aided clearance of extracellular α-synuclein prevents cell-to-cell aggregate transmission.
The tumor microenvironment of colorectal cancer: stromal TLR-4 expression as a potential prognostic marker.
Response of human islets to isolation stress and the effect of antioxidant treatment.
Response of human islets to isolation stress and the effect of antioxidant treatment.
Tessier S, Tran VA, Ottone OK, Novais EJ, Doolittle A, DiMuzio MJ, Shapiro IM, Risbud MV
Matrix biology : journal of the International Society for Matrix Biology 2020 May;87:94-111
Matrix biology : journal of the International Society for Matrix Biology 2020 May;87:94-111
Analysis of inflammatory cytokine expression in the urinary tract of BALB/c mice infected with Proteus (P.) mirabilis and enteroaggregative Escherichia (E.) coli (EAEC) strains.
Melendez-Avalos A, Sainz-Espuñes T, Castrillón-Rivera LE, Mendoza-Pérez F, Palma-Ramos A, Castañeda-Sánchez JI, Drago-Serrano EM
Folia microbiologica 2020 Feb;65(1):133-142
Folia microbiologica 2020 Feb;65(1):133-142
Acute changes of pro-inflammatory markers and corticosterone in experimental subarachnoid haemorrhage: A prerequisite for severity assessment.
Bach A, Conzen C, Schubert GA, Bleilevens C, Lindauer U
PloS one 2019;14(7):e0220467
PloS one 2019;14(7):e0220467
Silencing of A20 Aggravates Neuronal Death and Inflammation After Traumatic Brain Injury: A Potential Trigger of Necroptosis.
Bao Z, Fan L, Zhao L, Xu X, Liu Y, Chao H, Liu N, You Y, Liu Y, Wang X, Ji J
Frontiers in molecular neuroscience 2019;12:222
Frontiers in molecular neuroscience 2019;12:222
p16(Ink4a) deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
Novais EJ, Diekman BO, Shapiro IM, Risbud MV
Matrix biology : journal of the International Society for Matrix Biology 2019 Sep;82:54-70
Matrix biology : journal of the International Society for Matrix Biology 2019 Sep;82:54-70
Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice.
Adhikari N, Neupane S, Aryal YP, Choi M, Sohn WJ, Lee Y, Jung JK, Ha JH, Choi SY, Suh JY, Kim JY, Rho MC, Lee TH, Yamamoto H, An CH, Kim SH, An SY, Kim JY
Journal of periodontal research 2019 Oct;54(5):533-545
Journal of periodontal research 2019 Oct;54(5):533-545
Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense.
Chen M, Reed RR, Lane AP
Cell stem cell 2019 Oct 3;25(4):501-513.e5
Cell stem cell 2019 Oct 3;25(4):501-513.e5
Erlotinib overcomes paclitaxel-resistant cancer stem cells by blocking the EGFR-CREB/GRβ-IL-6 axis in MUC1-positive cervical cancer.
Lv Y, Cang W, Li Q, Liao X, Zhan M, Deng H, Li S, Jin W, Pang Z, Qiu X, Zhao K, Chen G, Qiu L, Huang L
Oncogenesis 2019 Nov 26;8(12):70
Oncogenesis 2019 Nov 26;8(12):70
Interleukin-12 and -23 blockade mitigates elastase-induced abdominal aortic aneurysm.
Yan H, Hu Y, Akk A, Ye K, Bacon J, Pham CTN
Scientific reports 2019 Jul 18;9(1):10447
Scientific reports 2019 Jul 18;9(1):10447
Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon.
Gurung S, Reuter N, Preno A, Dubaut J, Nadeau H, Hyatt K, Singleton K, Martin A, Parks WT, Papin JF, Myers DA
PLoS pathogens 2019 Jan;15(1):e1007507
PLoS pathogens 2019 Jan;15(1):e1007507
RNA inhibitors of nuclear proteins responsible for multiple organ dysfunction syndrome.
Urak KT, Blanco GN, Shubham S, Lin LH, Dassie JP, Thiel WH, Chen Y, Sonkar VK, Lei B, Murthy S, Gutierrez WR, Wilson ME, Stiber JA, Klesney-Tait J, Dayal S, Miller FJ Jr, Giangrande PH
Nature communications 2019 Jan 10;10(1):116
Nature communications 2019 Jan 10;10(1):116
Cell-type-specific role of lamin-B1 in thymus development and its inflammation-driven reduction in thymus aging.
Yue S, Zheng X, Zheng Y
Aging cell 2019 Aug;18(4):e12952
Aging cell 2019 Aug;18(4):e12952
BMP-2/7 heterodimer strongly induces bone regeneration in the absence of increased soft tissue inflammation.
Kaito T, Morimoto T, Mori Y, Kanayama S, Makino T, Takenaka S, Sakai Y, Otsuru S, Yoshioka Y, Yoshikawa H
The spine journal : official journal of the North American Spine Society 2018 Jan;18(1):139-146
The spine journal : official journal of the North American Spine Society 2018 Jan;18(1):139-146
Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α.
Wang Q, He Z, Huang M, Liu T, Wang Y, Xu H, Duan H, Ma P, Zhang L, Zamvil SS, Hidalgo J, Zhang Z, O'Rourke DM, Dahmane N, Brem S, Mou Y, Gong Y, Fan Y
Nature communications 2018 Feb 8;9(1):559
Nature communications 2018 Feb 8;9(1):559
Transgenic mice overexpressing human TNF-α experience early onset spontaneous intervertebral disc herniation in the absence of overt degeneration.
Gorth DJ, Shapiro IM, Risbud MV
Cell death & disease 2018 Dec 18;10(1):7
Cell death & disease 2018 Dec 18;10(1):7
Macropinocytosis of Nab-paclitaxel Drives Macrophage Activation in Pancreatic Cancer.
Cullis J, Siolas D, Avanzi A, Barui S, Maitra A, Bar-Sagi D
Cancer immunology research 2017 Mar;5(3):182-190
Cancer immunology research 2017 Mar;5(3):182-190
Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection.
Rossi AF, Cadamuro AC, Biselli-Périco JM, Leite KR, Severino FE, Reis PP, Cordeiro JA, Silva AE
Cellular microbiology 2016 Oct;18(10):1444-58
Cellular microbiology 2016 Oct;18(10):1444-58
Targeted CFTR gene disruption with zinc-finger nucleases in human intestinal epithelial cells induces oxidative stress and inflammation.
Kleme ML, Sané AT, Garofalo C, Levy E
The international journal of biochemistry & cell biology 2016 May;74:84-94
The international journal of biochemistry & cell biology 2016 May;74:84-94
AAV8-mediated expression of N-acetylglucosamine-1-phosphate transferase attenuates bone loss in a mouse model of mucolipidosis II.
Ko AR, Jin DK, Cho SY, Park SW, Przybylska M, Yew NS, Cheng SH, Kim JS, Kwak MJ, Kim SJ, Sohn YB
Molecular genetics and metabolism 2016 Apr;117(4):447-55
Molecular genetics and metabolism 2016 Apr;117(4):447-55
Porphyromonas gingivalis GroEL induces osteoclastogenesis of periodontal ligament cells and enhances alveolar bone resorption in rats.
Lin FY, Hsiao FP, Huang CY, Shih CM, Tsao NW, Tsai CS, Yang SF, Chang NC, Hung SL, Lin YW
PloS one 2014;9(7):e102450
PloS one 2014;9(7):e102450
Autophagy modulates SNCA/α-synuclein release, thereby generating a hostile microenvironment.
Poehler AM, Xiang W, Spitzer P, May VE, Meixner H, Rockenstein E, Chutna O, Outeiro TF, Winkler J, Masliah E, Klucken J
Autophagy 2014;10(12):2171-92
Autophagy 2014;10(12):2171-92
Adipocyte lipolysis-stimulated interleukin-6 production requires sphingosine kinase 1 activity.
Zhang W, Mottillo EP, Zhao J, Gartung A, VanHecke GC, Lee JF, Maddipati KR, Xu H, Ahn YH, Proia RL, Granneman JG, Lee MJ
The Journal of biological chemistry 2014 Nov 14;289(46):32178-32185
The Journal of biological chemistry 2014 Nov 14;289(46):32178-32185
Antibody-aided clearance of extracellular α-synuclein prevents cell-to-cell aggregate transmission.
Bae EJ, Lee HJ, Rockenstein E, Ho DH, Park EB, Yang NY, Desplats P, Masliah E, Lee SJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Sep 26;32(39):13454-69
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Sep 26;32(39):13454-69
The tumor microenvironment of colorectal cancer: stromal TLR-4 expression as a potential prognostic marker.
Cammarota R, Bertolini V, Pennesi G, Bucci EO, Gottardi O, Garlanda C, Laghi L, Barberis MC, Sessa F, Noonan DM, Albini A
Journal of translational medicine 2010 Nov 8;8:112
Journal of translational medicine 2010 Nov 8;8:112
Response of human islets to isolation stress and the effect of antioxidant treatment.
Bottino R, Balamurugan AN, Tse H, Thirunavukkarasu C, Ge X, Profozich J, Milton M, Ziegenfuss A, Trucco M, Piganelli JD
Diabetes 2004 Oct;53(10):2559-68
Diabetes 2004 Oct;53(10):2559-68
Response of human islets to isolation stress and the effect of antioxidant treatment.
Bottino R, Balamurugan AN, Tse H, Thirunavukkarasu C, Ge X, Profozich J, Milton M, Ziegenfuss A, Trucco M, Piganelli JD
Diabetes 2004 Oct;53(10):2559-68
Diabetes 2004 Oct;53(10):2559-68
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-6 Antibody [NB600-1131] - Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band 21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-6 Antibody [NB600-1131] - Western Blot: IL-6 Antibody [HRP] [NBP1-42762] - Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none. Image using the HRP form of this antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: IL-6 Antibody [NB600-1131] - GBM ECs express IL-6. Mouse GBM was induced by orthotopic injection of GL26 glioma cells into wild-type mouse. The brain sections that include normal brains and tumors were stained with anti-CD31, anti-IL-6, and anti-CSF-1 antibodies. Representative immunofluorescence images are shown. Right, enlarged area in normal and tumor tissues. Bar represents 50?um. Zoom-in factor: 4 Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-018-03050-0) licensed under a CC-BY licence.
