12345-1-AP

antibody from Proteintech Group

Targeting: DYNC1H1 CMT2O, DHC1, DNCH1, Dnchc1, DNCL, DNECL, HL-3, p22

Provider product page for 12345-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [27]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 12345-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12345-1-AP, RRID:AB_2261765
Product name: DYNC1H1 antibody
Antibody type: Polyclonal
Description: KD/KO validated DYNC1H1 antibody (Cat. #12345-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat, zebrafish, mouse and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat, Zebrafish
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

DYNC1H1 protein structure - 12345-1-AP shown in red.

Submitted references Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.

Kang Y, Yin S, Liu J, Jiang Y, Huang Z, Chen L, Shao L
Journal of hazardous materials 2022 Feb 15;424(Pt D):127660

Effect of conditional deletion of cytoplasmic dynein heavy chain DYNC1H1 on postnatal photoreceptors.

Dahl TM, Reed M, Gerstner CD, Ying G, Baehr W
PloS one 2021;16(3):e0248354

Conditional Deletion of Cytoplasmic Dynein Heavy Chain in Postnatal Photoreceptors.

Dahl TM, Reed M, Gerstner CD, Baehr W
Investigative ophthalmology & visual science 2021 Nov 1;62(14):23

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Wu X, Sheng H, Wang L, Xia P, Wang Y, Yu L, Lv W, Hu J
Aging 2021 Mar 26;13(7):10034-10057

Microfilaments and microtubules alternately coordinate the multi-step endosomal trafficking of Classical Swine Fever Virus.

Cheng Y, Lou JX, Liu CC, Liu YY, Chen XN, Liang XD, Zhang J, Yang Q, Go YY, Zhou B
Journal of virology 2021 Feb 24;

Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells.

Pedicini L, Wiktor SD, Simmons KJ, Money A, McKeown L
Scientific reports 2021 Feb 18;11(1):4054

A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.

Kesisova IA, Robinson BP, Spiliotis ET
The Journal of cell biology 2021 Feb 1;220(2)

Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.

Kumari A, Kumar C, Pergu R, Kumar M, Mahale SP, Wasnik N, Mylavarapu SVS
The Journal of cell biology 2021 Dec 6;220(12)

A Conserved Role for Vezatin Proteins in Cargo-Specific Regulation of Retrograde Axonal Transport.

Spinner MA, Pinter K, Drerup CM, Herman TG
Genetics 2020 Oct;216(2):431-445

Transgelin-2 and phosphoregulation of the LIC2 subunit of dynein govern mitotic spindle orientation.

Sharma A, Dagar S, Mylavarapu SVS
Journal of cell science 2020 Jun 23;133(12)

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Kodani A, Kenny C, Lai A, Gonzalez DM, Stronge E, Sejourne GM, Isacco L, Partlow JN, O'Donnell A, McWalter K, Byrne AB, Barkovich AJ, Yang E, Hill RS, Gawlinski P, Wiszniewski W, Cohen JS, Fatemi SA, Baranano KW, Sahin M, Vossler DG, Yuskaitis CJ, Walsh CA
Neuron 2020 Apr 22;106(2):246-255.e6

Enhancing the retrograde axonal transport by curcumin promotes autophagic flux in N2a/APP695swe cells.

Liang J, Zhou F, Xiong X, Zhang X, Li S, Li X, Gao M, Li Y
Aging 2019 Sep 6;11(17):7036-7050

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

So C, Seres KB, Steyer AM, Mönnich E, Clift D, Pejkovska A, Möbius W, Schuh M
Science (New York, N.Y.) 2019 Jun 28;364(6447)

Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement.

Wang Q, Tian J, Chen H, Du H, Guo L
Neurobiology of disease 2019 Jul;127:410-418

Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells.

Franchini DM, Lanvin O, Tosolini M, Patras de Campaigno E, Cammas A, Péricart S, Scarlata CM, Lebras M, Rossi C, Ligat L, Pont F, Arimondo PB, Laurent C, Ayyoub M, Despas F, Lapeyre-Mestre M, Millevoi S, Fournié JJ
Cell reports 2019 Jan 2;26(1):94-107.e7

EB1 and cytoplasmic dynein mediate protrusion dynamics for efficient 3-dimensional cell migration.

Jayatilaka H, Giri A, Karl M, Aifuwa I, Trenton NJ, Phillip JM, Khatau S, Wirtz D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Mar;32(3):1207-1221

Palladin is a novel microtubule-associated protein responsible for spindle orientation.

Zhang X, Chen X, Liu J, Xu X, Zhang Y, Ruan Z, Xie Y, Huang Q, Yin T, Chen Z, Chen S
Scientific reports 2017 Sep 18;7(1):11806

Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons.

Drerup CM, Herbert AL, Monk KR, Nechiporuk AV
eLife 2017 Apr 17;6

Dynein Light Intermediate Chain 2 Facilitates the Metaphase to Anaphase Transition by Inactivating the Spindle Assembly Checkpoint.

Mahale SP, Sharma A, Mylavarapu SV
PloS one 2016;11(7):e0159646

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

Inaba H, Goto H, Kasahara K, Kumamoto K, Yonemura S, Inoko A, Yamano S, Wanibuchi H, He D, Goshima N, Kiyono T, Hirotsune S, Inagaki M
The Journal of cell biology 2016 Feb 15;212(4):409-23

Knockdown of Dynamitin in testes significantly decreased male fertility in Drosophila melanogaster.

Wu CH, Zong Q, Du AL, Zhang W, Yao HC, Yu XQ, Wang YF
Developmental biology 2016 Dec 1;420(1):79-89

Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis.

Gholkar AA, Senese S, Lo YC, Capri J, Deardorff WJ, Dharmarajan H, Contreras E, Hodara E, Whitelegge JP, Jackson PK, Torres JZ
Cell cycle (Georgetown, Tex.) 2015;14(7):1116-25

Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.

Renard HF, Simunovic M, Lemière J, Boucrot E, Garcia-Castillo MD, Arumugam S, Chambon V, Lamaze C, Wunder C, Kenworthy AK, Schmidt AA, McMahon HT, Sykes C, Bassereau P, Johannes L
Nature 2015 Jan 22;517(7535):493-6

A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region.

Schroeder CM, Ostrem JM, Hertz NT, Vale RD
eLife 2014 Oct 1;3:e03351

Depletion of pre-mRNA splicing factor Cdc5L inhibits mitotic progression and triggers mitotic catastrophe.

Mu R, Wang YB, Wu M, Yang Y, Song W, Li T, Zhang WN, Tan B, Li AL, Wang N, Xia Q, Gong WL, Wang CG, Zhou T, Guo N, Sang ZH, Li HY
Cell death & disease 2014 Mar 27;5:e1151

Proteome analysis of the sera from Chinese Parkinson's disease patients.

Zhao X, Xiao WZ, Pu XP, Zhong LJ
Neuroscience letters 2010 Jul 26;479(2):175-9

Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development.

Insinna C, Baye LM, Amsterdam A, Besharse JC, Link BA
Neural development 2010 Apr 22;5:12

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 12345-1-AP(KIAA0325 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using DYNC1H1 antibody 12345-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 12345-1-AP(KIAA0325 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue