HPA022140

antibody from Atlas Antibodies

Targeting: PFAS FGARAT, GATD8, KIAA0361, PURL

Antibody data

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Validation data

Product number: HPA022140 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA022140, RRID:AB_1855232
Product name: Anti-PFAS
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: LFGCPLLLDDVARESWLLPGSNDLLLEVGPRLNFS TPTSTNIVSVCRATGLGPVDRVETTRRYRLSFAHP PSAEVEAIALATL
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

PFAS protein structure - HPA022140 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human lymph node and skeletal muscle tissues using Anti-PFAS antibody. Corresponding PFAS RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human kidney, lymph node, skeletal muscle and testis using Anti-PFAS antibody HPA022140 (A) shows similar protein distribution across tissues to independent antibody HPA022886 (B).
Antibody #2 product nr: HPA022886
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human lymph node shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skeletal muscle shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis using Anti-PFAS antibody HPA022140.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney using Anti-PFAS antibody HPA022140.
Sample type: HUMAN