AP10957b

antibody from Abgent

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP10957b

Provider

Abgent

Proper citation

Abgent Cat#AP10957b, RRID:AB_10818583

Product name

IL18 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of IL18 (arrow) using rabbit polyclonal IL18 Antibody (Center) (Cat. #AP10957b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL18 gene (Lane 2) .

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

IL18 Antibody (Center) (Cat. #AP10957b) western blot analysis in MDA-MB468 cell line lysates (35ug/lane).This demonstrates the IL18 antibody detected the IL18 protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of IL18 Antibody (Center)(Cat. #AP10957b) with Hela cell followed by Alexa Fluor4d?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

IL18 antibody (Center) (Cat. #AP10957b) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL18 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50