Antibody data
- Antibody Data
- Antigen structure
- References [43]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [6]
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- Product number
- PA3-514 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Glucocorticoid Receptor beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA3-514 detects glucocorticoid receptor (GR) beta from human cells.
- Concentration
- Conc. Not Determined
Submitted references Glucocorticoid receptor GRβ regulates glucocorticoid-induced ocular hypertension in mice.
LPS regulates the expression of glucocorticoid receptor α and β isoforms and induces a selective glucocorticoid resistance in vitro.
Annexin A1 in blood mononuclear cells from patients with coronary artery disease: Its association with inflammatory status and glucocorticoid sensitivity.
Overexpression of GRß in colonic mucosal cell line partly reflects altered gene expression in colonic mucosa of patients with inflammatory bowel disease.
Effects of maternal dexamethasone treatment early in pregnancy on glucocorticoid receptors in the ovine placenta.
Effect of ionizing radiation on human skeletal muscle precursor cells.
Deficient glucocorticoid induction of anti-inflammatory genes in nasal polyp fibroblasts of asthmatic patients with and without aspirin intolerance.
Septic serum induces glucocorticoid resistance and modifies the expression of glucocorticoid isoforms receptors: a prospective cohort study and in vitro experimental assay.
Glucocorticoid receptor and molecular chaperones in the pathogenesis of adrenal incidentalomas: potential role of reduced sensitivity to glucocorticoids.
Glucocorticoid receptor β and histone deacetylase 1 and 2 expression in the airways of severe asthma.
Expression of glucocorticoid receptors in the regenerating human skeletal muscle.
Expression of glucocorticoid receptors in the regenerating human skeletal muscle.
Effects of methotrexate on the expression of the translational isoforms of glucocorticoid receptors α and β: correlation with methotrexate efficacy in rheumatoid arthritis patients.
The dominant negative β isoform of the glucocorticoid receptor is uniquely expressed in erythroid cells expanded from polycythemia vera patients.
Enhanced neutrophil expression of annexin-1 in coronary artery disease.
Expression and distribution of glucocorticoid receptor isoforms in eosinophilic chronic rhinosinusitis.
HIF-1alpha response to hypoxia is functionally separated from the glucocorticoid stress response in the in vitro regenerating human skeletal muscle.
Glucocorticoids with different chemical structures but similar glucocorticoid receptor potency regulate subsets of common and unique genes in human trabecular meshwork cells.
Expression and distribution of glucocorticoid receptors in the ovine fetal adrenal cortex: effect of long-term hypoxia.
Changes of glucocorticoid receptor expression in the nasal polyps of patients with chronic sinusitis following treatment with glucocorticoid.
Human glucocorticoid receptor beta binds RU-486 and is transcriptionally active.
Nitric oxide up-regulates the glucocorticoid receptor and blunts the inflammatory reaction in porcine endotoxin sepsis.
Dexamethasone inhibition of trabecular meshwork cell phagocytosis and its modulation by glucocorticoid receptor beta.
Distribution of glucocorticoid receptor alpha and beta subtypes in the idiopathic inflammatory myopathies.
The differential expression of corticosteroid receptor isoforms in corticosteroid-resistant and -sensitive patients with rheumatoid arthritis.
CD38 expression is insensitive to steroid action in cells treated with tumor necrosis factor-alpha and interferon-gamma by a mechanism involving the up-regulation of the glucocorticoid receptor beta isoform.
The mitochondrion as a primary site of action of steroid and thyroid hormones: presence and action of steroid and thyroid hormone receptors in mitochondria of animal cells.
Expression of glucocorticoid receptors in non-neoplastic lymphoid follicles and B cell type malignant lymphomas.
Expression and regulation of glucocorticoid receptor in human placental villous fibroblasts.
The human glucocorticoid receptor (hGR) beta isoform suppresses the transcriptional activity of hGRalpha by interfering with formation of active coactivator complexes.
Glucocorticoid receptor subunit expression in adenotonsillar tissue of children with obstructive sleep apnea.
Regulation of glucocorticoid responsiveness in glaucomatous trabecular meshwork cells by glucocorticoid receptor-beta.
Glucocorticoid receptor isoforms in human hepatocarcinoma HepG2 and SaOS-2 osteosarcoma cells: presence of glucocorticoid receptor alpha in mitochondria and of glucocorticoid receptor beta in nucleoli.
Glucocorticoid receptor alpha, beta and gamma expression vs in vitro glucocorticod resistance in childhood leukemia.
Steroid-responsive sequences in the human glucocorticoid receptor gene 1A promoter.
Serine-arginine-rich protein p30 directs alternative splicing of glucocorticoid receptor pre-mRNA to glucocorticoid receptor beta in neutrophils.
Effect of labor on glucocorticoid receptor (GR(Total), GRalpha, and GRbeta) proteins in ovine intrauterine tissues.
Increased glucocorticoid receptor Beta expression converts mouse hybridoma cells to a corticosteroid-insensitive phenotype.
Multiple promoters exist in the human GR gene, one of which is activated by glucocorticoids.
Western blotting for detection of glucocorticoid receptors in the brain and pituitary gland from adrenal intact pigs.
Expression and subcellular distribution of the beta-isoform of the human glucocorticoid receptor.
Binding of site-directed monoclonal antibodies to an epitope located in the A/B region (amino acids 140-154) of human estrogen receptor-induced conformational changes in an epitope in the DNA-binding domain.
The human glucocorticoid receptor beta isoform. Expression, biochemical properties, and putative function.
Patel GC, Liu Y, Millar JC, Clark AF
Scientific reports 2018 Jan 16;8(1):862
Scientific reports 2018 Jan 16;8(1):862
LPS regulates the expression of glucocorticoid receptor α and β isoforms and induces a selective glucocorticoid resistance in vitro.
Molina ML, Guerrero J, Cidlowski JA, Gatica H, Goecke A
Journal of inflammation (London, England) 2017;14:22
Journal of inflammation (London, England) 2017;14:22
Annexin A1 in blood mononuclear cells from patients with coronary artery disease: Its association with inflammatory status and glucocorticoid sensitivity.
Bergström I, Lundberg AK, Jönsson S, Särndahl E, Ernerudh J, Jonasson L
PloS one 2017;12(3):e0174177
PloS one 2017;12(3):e0174177
Overexpression of GRß in colonic mucosal cell line partly reflects altered gene expression in colonic mucosa of patients with inflammatory bowel disease.
Nagy Z, Acs B, Butz H, Feldman K, Marta A, Szabo PM, Baghy K, Pazmany T, Racz K, Liko I, Patocs A
The Journal of steroid biochemistry and molecular biology 2016 Jan;155(Pt A):76-84
The Journal of steroid biochemistry and molecular biology 2016 Jan;155(Pt A):76-84
Effects of maternal dexamethasone treatment early in pregnancy on glucocorticoid receptors in the ovine placenta.
Shang H, Meng W, Sloboda DM, Li S, Ehrlich L, Plagemann A, Dudenhausen JW, Henrich W, Newnham JP, Challis JR, Braun T
Reproductive sciences (Thousand Oaks, Calif.) 2015 May;22(5):534-44
Reproductive sciences (Thousand Oaks, Calif.) 2015 May;22(5):534-44
Effect of ionizing radiation on human skeletal muscle precursor cells.
Jurdana M, Cemazar M, Pegan K, Mars T
Radiology and oncology 2013;47(4):376-81
Radiology and oncology 2013;47(4):376-81
Deficient glucocorticoid induction of anti-inflammatory genes in nasal polyp fibroblasts of asthmatic patients with and without aspirin intolerance.
Fernández-Bertolín L, Mullol J, Fuentes-Prado M, Alobid I, Roca-Ferrer J, Picado C, Pujols L
The Journal of allergy and clinical immunology 2013 Nov;132(5):1243-1246.e12
The Journal of allergy and clinical immunology 2013 Nov;132(5):1243-1246.e12
Septic serum induces glucocorticoid resistance and modifies the expression of glucocorticoid isoforms receptors: a prospective cohort study and in vitro experimental assay.
Guerrero J, Gatica HA, Rodríguez M, Estay R, Goecke IA
Critical care (London, England) 2013 Jun 12;17(3):R107
Critical care (London, England) 2013 Jun 12;17(3):R107
Glucocorticoid receptor and molecular chaperones in the pathogenesis of adrenal incidentalomas: potential role of reduced sensitivity to glucocorticoids.
Damjanovic SS, Antic JA, Ilic BB, Cokic BB, Ivovic M, Ognjanovic SI, Isailovic TV, Popovic BM, Bozic IB, Tatic S, Matic G, Todorovic VN, Paunovic I
Molecular medicine (Cambridge, Mass.) 2013 Jan 22;18(1):1456-65
Molecular medicine (Cambridge, Mass.) 2013 Jan 22;18(1):1456-65
Glucocorticoid receptor β and histone deacetylase 1 and 2 expression in the airways of severe asthma.
Butler CA, McQuaid S, Taggart CC, Weldon S, Carter R, Skibinski G, Warke TJ, Choy DF, McGarvey LP, Bradding P, Arron JR, Heaney LG
Thorax 2012 May;67(5):392-8
Thorax 2012 May;67(5):392-8
Expression of glucocorticoid receptors in the regenerating human skeletal muscle.
Filipović D, Pirkmajer S, Mis K, Mars T, Grubic Z
Physiological research 2011;60(Suppl 1):S147-54
Physiological research 2011;60(Suppl 1):S147-54
Expression of glucocorticoid receptors in the regenerating human skeletal muscle.
Filipović D, Pirkmajer S, Mis K, Mars T, Grubic Z
Physiological research 2011;60 Suppl 1:S147-54
Physiological research 2011;60 Suppl 1:S147-54
Effects of methotrexate on the expression of the translational isoforms of glucocorticoid receptors α and β: correlation with methotrexate efficacy in rheumatoid arthritis patients.
Gatica H, Aliste M, Guerrero J, Goecke IA
Rheumatology (Oxford, England) 2011 Sep;50(9):1665-71
Rheumatology (Oxford, England) 2011 Sep;50(9):1665-71
The dominant negative β isoform of the glucocorticoid receptor is uniquely expressed in erythroid cells expanded from polycythemia vera patients.
Varricchio L, Masselli E, Alfani E, Battistini A, Migliaccio G, Vannucchi AM, Zhang W, Rondelli D, Godbold J, Ghinassi B, Whitsett C, Hoffman R, Migliaccio AR
Blood 2011 Jul 14;118(2):425-36
Blood 2011 Jul 14;118(2):425-36
Enhanced neutrophil expression of annexin-1 in coronary artery disease.
Särndahl E, Bergström I, Nijm J, Forslund T, Perretti M, Jonasson L
Metabolism: clinical and experimental 2010 Mar;59(3):433-40
Metabolism: clinical and experimental 2010 Mar;59(3):433-40
Expression and distribution of glucocorticoid receptor isoforms in eosinophilic chronic rhinosinusitis.
Takeda K, Takeno S, Hirakawa K, Ishino T
Auris, nasus, larynx 2010 Dec;37(6):700-7
Auris, nasus, larynx 2010 Dec;37(6):700-7
HIF-1alpha response to hypoxia is functionally separated from the glucocorticoid stress response in the in vitro regenerating human skeletal muscle.
Pirkmajer S, Filipovic D, Mars T, Mis K, Grubic Z
American journal of physiology. Regulatory, integrative and comparative physiology 2010 Dec;299(6):R1693-700
American journal of physiology. Regulatory, integrative and comparative physiology 2010 Dec;299(6):R1693-700
Glucocorticoids with different chemical structures but similar glucocorticoid receptor potency regulate subsets of common and unique genes in human trabecular meshwork cells.
Nehmé A, Lobenhofer EK, Stamer WD, Edelman JL
BMC medical genomics 2009 Sep 10;2:58
BMC medical genomics 2009 Sep 10;2:58
Expression and distribution of glucocorticoid receptors in the ovine fetal adrenal cortex: effect of long-term hypoxia.
Root B, Abrassart J, Myers DA, Monau T, Ducsay CA
Reproductive sciences (Thousand Oaks, Calif.) 2008 May;15(5):517-28
Reproductive sciences (Thousand Oaks, Calif.) 2008 May;15(5):517-28
Changes of glucocorticoid receptor expression in the nasal polyps of patients with chronic sinusitis following treatment with glucocorticoid.
Watanabe S, Suzaki H
In vivo (Athens, Greece) 2008 Jan-Feb;22(1):37-42
In vivo (Athens, Greece) 2008 Jan-Feb;22(1):37-42
Human glucocorticoid receptor beta binds RU-486 and is transcriptionally active.
Lewis-Tuffin LJ, Jewell CM, Bienstock RJ, Collins JB, Cidlowski JA
Molecular and cellular biology 2007 Mar;27(6):2266-82
Molecular and cellular biology 2007 Mar;27(6):2266-82
Nitric oxide up-regulates the glucocorticoid receptor and blunts the inflammatory reaction in porcine endotoxin sepsis.
Da J, Chen L, Hedenstierna G
Critical care medicine 2007 Jan;35(1):26-32
Critical care medicine 2007 Jan;35(1):26-32
Dexamethasone inhibition of trabecular meshwork cell phagocytosis and its modulation by glucocorticoid receptor beta.
Zhang X, Ognibene CM, Clark AF, Yorio T
Experimental eye research 2007 Feb;84(2):275-84
Experimental eye research 2007 Feb;84(2):275-84
Distribution of glucocorticoid receptor alpha and beta subtypes in the idiopathic inflammatory myopathies.
De Bleecker JL, De Paepe B, Vervaet VL, Arys B, Creus KK, Werbrouck BF, Martin JJ
Neuromuscular disorders : NMD 2007 Feb;17(2):186-93
Neuromuscular disorders : NMD 2007 Feb;17(2):186-93
The differential expression of corticosteroid receptor isoforms in corticosteroid-resistant and -sensitive patients with rheumatoid arthritis.
Kozaci DL, Chernajovsky Y, Chikanza IC
Rheumatology (Oxford, England) 2007 Apr;46(4):579-85
Rheumatology (Oxford, England) 2007 Apr;46(4):579-85
CD38 expression is insensitive to steroid action in cells treated with tumor necrosis factor-alpha and interferon-gamma by a mechanism involving the up-regulation of the glucocorticoid receptor beta isoform.
Tliba O, Cidlowski JA, Amrani Y
Molecular pharmacology 2006 Feb;69(2):588-96
Molecular pharmacology 2006 Feb;69(2):588-96
The mitochondrion as a primary site of action of steroid and thyroid hormones: presence and action of steroid and thyroid hormone receptors in mitochondria of animal cells.
Psarra AM, Solakidi S, Sekeris CE
Molecular and cellular endocrinology 2006 Feb 26;246(1-2):21-33
Molecular and cellular endocrinology 2006 Feb 26;246(1-2):21-33
Expression of glucocorticoid receptors in non-neoplastic lymphoid follicles and B cell type malignant lymphomas.
Okazaki SI, Yamakawa M, Maeda K, Ohta N, Aoyagi M
Journal of clinical pathology 2006 Apr;59(4):410-6
Journal of clinical pathology 2006 Apr;59(4):410-6
Expression and regulation of glucocorticoid receptor in human placental villous fibroblasts.
Lee MJ, Wang Z, Yee H, Ma Y, Swenson N, Yang L, Kadner SS, Baergen RN, Logan SK, Garabedian MJ, Guller S
Endocrinology 2005 Nov;146(11):4619-26
Endocrinology 2005 Nov;146(11):4619-26
The human glucocorticoid receptor (hGR) beta isoform suppresses the transcriptional activity of hGRalpha by interfering with formation of active coactivator complexes.
Charmandari E, Chrousos GP, Ichijo T, Bhattacharyya N, Vottero A, Souvatzoglou E, Kino T
Molecular endocrinology (Baltimore, Md.) 2005 Jan;19(1):52-64
Molecular endocrinology (Baltimore, Md.) 2005 Jan;19(1):52-64
Glucocorticoid receptor subunit expression in adenotonsillar tissue of children with obstructive sleep apnea.
Goldbart AD, Veling MC, Goldman JL, Li RC, Brittian KR, Gozal D
Pediatric research 2005 Feb;57(2):232-6
Pediatric research 2005 Feb;57(2):232-6
Regulation of glucocorticoid responsiveness in glaucomatous trabecular meshwork cells by glucocorticoid receptor-beta.
Zhang X, Clark AF, Yorio T
Investigative ophthalmology & visual science 2005 Dec;46(12):4607-16
Investigative ophthalmology & visual science 2005 Dec;46(12):4607-16
Glucocorticoid receptor isoforms in human hepatocarcinoma HepG2 and SaOS-2 osteosarcoma cells: presence of glucocorticoid receptor alpha in mitochondria and of glucocorticoid receptor beta in nucleoli.
Psarra AM, Solakidi S, Trougakos IP, Margaritis LH, Spyrou G, Sekeris CE
The international journal of biochemistry & cell biology 2005 Dec;37(12):2544-58
The international journal of biochemistry & cell biology 2005 Dec;37(12):2544-58
Glucocorticoid receptor alpha, beta and gamma expression vs in vitro glucocorticod resistance in childhood leukemia.
Haarman EG, Kaspers GJ, Pieters R, Rottier MM, Veerman AJ
Leukemia 2004 Mar;18(3):530-7
Leukemia 2004 Mar;18(3):530-7
Steroid-responsive sequences in the human glucocorticoid receptor gene 1A promoter.
Geng CD, Vedeckis WV
Molecular endocrinology (Baltimore, Md.) 2004 Apr;18(4):912-24
Molecular endocrinology (Baltimore, Md.) 2004 Apr;18(4):912-24
Serine-arginine-rich protein p30 directs alternative splicing of glucocorticoid receptor pre-mRNA to glucocorticoid receptor beta in neutrophils.
Xu Q, Leung DY, Kisich KO
The Journal of biological chemistry 2003 Jul 18;278(29):27112-8
The Journal of biological chemistry 2003 Jul 18;278(29):27112-8
Effect of labor on glucocorticoid receptor (GR(Total), GRalpha, and GRbeta) proteins in ovine intrauterine tissues.
Gupta S, Gyomorey S, Lye SJ, Gibb W, Challis JR
Journal of the Society for Gynecologic Investigation 2003 Apr;10(3):136-44
Journal of the Society for Gynecologic Investigation 2003 Apr;10(3):136-44
Increased glucocorticoid receptor Beta expression converts mouse hybridoma cells to a corticosteroid-insensitive phenotype.
Hauk PJ, Goleva E, Strickland I, Vottero A, Chrousos GP, Kisich KO, Leung DY
American journal of respiratory cell and molecular biology 2002 Sep;27(3):361-7
American journal of respiratory cell and molecular biology 2002 Sep;27(3):361-7
Multiple promoters exist in the human GR gene, one of which is activated by glucocorticoids.
Breslin MB, Geng CD, Vedeckis WV
Molecular endocrinology (Baltimore, Md.) 2001 Aug;15(8):1381-95
Molecular endocrinology (Baltimore, Md.) 2001 Aug;15(8):1381-95
Western blotting for detection of glucocorticoid receptors in the brain and pituitary gland from adrenal intact pigs.
Weaver SA, Schaefer AL, Dixon WT
Brain research 2000 Jun 30;869(1-2):130-6
Brain research 2000 Jun 30;869(1-2):130-6
Expression and subcellular distribution of the beta-isoform of the human glucocorticoid receptor.
Oakley RH, Webster JC, Sar M, Parker CR Jr, Cidlowski JA
Endocrinology 1997 Nov;138(11):5028-38
Endocrinology 1997 Nov;138(11):5028-38
Binding of site-directed monoclonal antibodies to an epitope located in the A/B region (amino acids 140-154) of human estrogen receptor-induced conformational changes in an epitope in the DNA-binding domain.
Traish AM, Pavao M
Steroids 1996 Sep;61(9):549-56
Steroids 1996 Sep;61(9):549-56
The human glucocorticoid receptor beta isoform. Expression, biochemical properties, and putative function.
Oakley RH, Sar M, Cidlowski JA
The Journal of biological chemistry 1996 Apr 19;271(16):9550-9
The Journal of biological chemistry 1996 Apr 19;271(16):9550-9
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Western blot of hGR beta transfected COS-7 whole cell extract using Product # PA3-514.
Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
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- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
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- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 1 Western blot analysis of glucocorticoid receptor alpha and beta isoforms of peripheral blood mononuclear cells from septic patients . GRalpha and GRbeta cell expression were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on ICU admission (first day in the ICU) and on the day of hospital discharge, which was considered as the control situation (free of sepsis). The quantification of the western blot analysis of (A) GRalpha and (B) GRbeta is shown. The values were normalized to beta actin expression and are expressed as percentage values of the control (GR expression on the day of hospital discharge). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). (C) Representative western blot analysis of GRalphaGRbeta and beta actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 2 Western blot analysis of glucocorticoid receptor alpha and beta isoforms of peripheral blood mononuclear cells from septic patients on first and third day in the intensive care unit . The GRalpha and GRbeta cell expressions were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on the first and third day in ICU. The quantification of the western blot analysis of (A) GRalpha and (B) GRbeta are shown. The values were normalized to beta actin expression and are expressed as percentage values of the GR expression on the first day in ICU. Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). ns = not statistically significant. (C) Representative western blot analysis of GC receptors alphabeta and beta actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 4 Effect of human septic serum on glucocorticoid receptor isoform expression in different cell lines . CEM, Raji and K562 cell lines were cultured in the presence of the patients' septic serum and serum obtained from the control group at 10% final concentration in the culture medium. After 48 h in culture, the GR protein expression was analyzed by western blotting with specific antibodies for GRalpha and beta isoforms. The quantification of the western blot analysis of (A) GRalpha and (B) GRbeta are shown ( n = 9). The values were normalized to beta actin expression and are expressed as percentage values of the control (GR cell expression cultured in the presence of control serum). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). Representative western blot analysis of GRalpha, beta and beta actin are shown for each cell line. CS, control serum; GR, glucocorticoid receptor; SS, septic serum.