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LearnLS-C203254
antibody from LSBio
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [3]
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Validation data
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- Product number
- LS-C203254 - Provider product page
- Provider
- LSBio
- Product name
- IL8 / Interleukin 8 Antibody (clone 1A7C8) LS-C203254
- Antibody type
- Monoclonal
- Description
- Protein A purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1A7C8
- Storage
- Store at -20°C. Long term: aliquot and store at -20°C. Reconstituted: store at 2°C to 8°C for up to 3 weeks. Avoid freeze/thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of human recombinant IL-8 fusion protein using Human IL-8 Antibody (1A7C8), mAb, Mouse The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 36 KD Observed Size: 36 KD
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of human recombinant IL-8 fusion protein using Human IL-8 Antibody (1A7C8), mAb, Mouse The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 36 KD Observed Size: 36 KD
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2. Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 2D11C10, 3D4C3 or 4H7C7) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5. Stop the reaction with 1.0 N HCl and read the plate at 450nm.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2. Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 2D11C10, 3D4C3 or 4H7C7) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5. Stop the reaction with 1.0 N HCl and read the plate at 450nm.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2. Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 2D11C10, 3D4C3 or 4H7C7) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5. Stop the reaction with 1.0 N HCl and read the plate at 450nm.
