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LearnAB-208-NA
antibody from Novus Biologicals
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-208-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CXCL8/IL-8 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) GRO alpha , rhGRO beta , and rhGRO gamma is observed, and less than 1% cross-reactivity with recombinant feline IL-8/CXCL8, recombinant porcine IL-8/CXCL8, and recombinant canine IL-8/CXCL8 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in arthritis.
Evidence for chemokine synergy during neutrophil migration in ARDS.
Salmonella enteritidis Effector AvrA Stabilizes Intestinal Tight Junctions via the JNK Pathway.
Expression and regulation of chemokines in murine and human type 1 diabetes.
Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
Tumor-derived chemokine MCP-1/CCL2 is sufficient for mediating tumor tropism of adoptively transferred T cells.
Tumor-derived chemokine MCP-1/CCL2 is sufficient for mediating tumor tropism of adoptively transferred T cells.
The effect of respiratory synctial virus on chemokine release by differentiated airway epithelium.
The effect of respiratory synctial virus on chemokine release by differentiated airway epithelium.
Angelini A, Miyabe Y, Newsted D, Kwan BH, Miyabe C, Kelly RL, Jamy MN, Luster AD, Wittrup KD
Nature communications 2018 Apr 13;9(1):1461
Nature communications 2018 Apr 13;9(1):1461
Evidence for chemokine synergy during neutrophil migration in ARDS.
Williams AE, José RJ, Mercer PF, Brealey D, Parekh D, Thickett DR, O'Kane C, McAuley DF, Chambers RC
Thorax 2017 Jan;72(1):66-73
Thorax 2017 Jan;72(1):66-73
Salmonella enteritidis Effector AvrA Stabilizes Intestinal Tight Junctions via the JNK Pathway.
Lin Z, Zhang YG, Xia Y, Xu X, Jiao X, Sun J
The Journal of biological chemistry 2016 Dec 23;291(52):26837-26849
The Journal of biological chemistry 2016 Dec 23;291(52):26837-26849
Expression and regulation of chemokines in murine and human type 1 diabetes.
Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
Diabetes 2012 Feb;61(2):436-46
Diabetes 2012 Feb;61(2):436-46
Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E
Nature medicine 2011 Oct 30;17(11):1498-503
Nature medicine 2011 Oct 30;17(11):1498-503
Tumor-derived chemokine MCP-1/CCL2 is sufficient for mediating tumor tropism of adoptively transferred T cells.
Brown CE, Vishwanath RP, Aguilar B, Starr R, Najbauer J, Aboody KS, Jensen MC
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 1;179(5):3332-41
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 1;179(5):3332-41
Tumor-derived chemokine MCP-1/CCL2 is sufficient for mediating tumor tropism of adoptively transferred T cells.
Brown CE, Vishwanath RP, Aguilar B, Starr R, Najbauer J, Aboody KS, Jensen MC
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 1;179(5):3332-41
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 1;179(5):3332-41
The effect of respiratory synctial virus on chemokine release by differentiated airway epithelium.
Mellow TE, Murphy PC, Carson JL, Noah TL, Zhang L, Pickles RJ
Experimental lung research 2004 Jan-Feb;30(1):43-57
Experimental lung research 2004 Jan-Feb;30(1):43-57
The effect of respiratory synctial virus on chemokine release by differentiated airway epithelium.
Mellow TE, Murphy PC, Carson JL, Noah TL, Zhang L, Pickles RJ
Experimental lung research 2004 Jan-Feb;30(1):43-57
Experimental lung research 2004 Jan-Feb;30(1):43-57
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody. Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-8/CXCL8 Polyclonal Antibody (Catalog # AB-208-NA). The ND50 is typically 5-20 µg/mL.
