MAB16081

antibody from Novus Biologicals

Targeting: CXCL8 3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB16081

Provider

Novus Biologicals

Product name

Mouse Monoclonal CXCL8/IL-8 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects canine IL-8/CXCL8 in ELISAs. In ELISAs, no cross-reactivity with recombinant feline IL-8/CXCL8, recombinant human CXCL8/IL-8, recombinant porcine CXCL8/IL-8, recombinant canine (rca) IL-1 beta/IL-1F2, or rcaCXCL6/IL-6 is observed.

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

500 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chemotaxis Induced by CXCL8/IL-8 and Neutralization by Canine CXCL8/IL-8 Antibody. Recombinant Canine CXCL8/IL-8 (Catalog # 1608-CL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CXCL8/IL-8 (10 ng/mL) is neutralized (green line) by increasing concentrations of Canine CXCL8/IL-8 Monoclonal Antibody (Catalog # MAB16081). The ND₅₀ is typically 3-12 µg/mL.