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LearnAF535
antibody from R&D Systems
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF535 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine IL-8/CXCL8 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human IL-8 is observed.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
CAA43461- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Exposure to hog barn dust alters airway epithelial ciliary beating.
Wyatt TA, Sisson JH, Von Essen SG, Poole JA, Romberger DJ
The European respiratory journal 2008 Jun;31(6):1249-55
The European respiratory journal 2008 Jun;31(6):1249-55
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine CXCL8/IL-8 Antibody. Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF535). The ND50 is typically 1.5-7.5 µg/mL.
