358408

antibody from BioLegend

Targeting: TNFRSF1B CD120b, p75, TNF-R-II, TNF-R75, TNFBR, TNFR2, TNFR80

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

358408

Provider

BioLegend

Proper citation

BioLegend Cat#358408, RRID:AB_2563224

Product name

Ultra-LEAF™ Purified anti-human CD120b

Antibody type

Monoclonal

Antigen

Partial CD120b recombinant protein expressed in 293E cells (22-211aa).

Description

CD120b, also known as TNFRII or TNFRSF1B, belongs to the TNF receptor superfamily and can interact with ligands TNF-α and TNF-β (also known as LT-α). It is a 75 kD type I transmembrane protein expressed on lymphocytes, monocytes, and granulocytes. When present in a heterocomplex with CD120a, it induces the recruitment of anti-apoptotic molecules, c-IAP1 and c-IAP2 that possess E3 ubiquitin ligase activity. Mutations in CD120b are associated with TNF-associated periodic syndrome (TRAPS) or periodic fever syndrome.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

3G7A02

Vial size

500 µg

Concentration

1.0 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Blocking/Neutralizing

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

TNF RII/TNFRSF1B Inhibition of TNF‑alpha-induced Cytotoxicity and Neutralization by Human CD120b Antibody. Recombinant Human sTNF RII (Catalog # 591602) inhibits Recombinant Human TNF‑alpha (Catalog # 570102) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (black line). Inhibition of Recombinant Human TNF‑alpha (0.25 ng/mL) activity elicited by Recombinant Human sTNF RII (0.4 µg/mL) is neutralized by increasing concentrations of Human CD120b Monoclonal Antibody (Red line) in the presence of the metabolic inhibitor actinomycin D (1 µg/ml). The ED₅₀ is 0.5-1.5 µg/ml.

Conjugate

Unconjugated