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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- AF3297 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal XPE/DDB2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human XPE/DDB2 in Western blots.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism.
Human CRL4(DDB2) ubiquitin ligase preferentially regulates post-repair chromatin restoration of H3K56Ac through recruitment of histone chaperon CAF-1.
Lai TC, Hu MC
Scientific reports 2019 Mar 28;9(1):5304
Scientific reports 2019 Mar 28;9(1):5304
Human CRL4(DDB2) ubiquitin ligase preferentially regulates post-repair chromatin restoration of H3K56Ac through recruitment of histone chaperon CAF-1.
Zhu Q, Wei S, Sharma N, Wani G, He J, Wani AA
Oncotarget 2017 Nov 28;8(61):104525-104542
Oncotarget 2017 Nov 28;8(61):104525-104542
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human XPE/DDB2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPE/DDB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3297) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPE/DDB2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
