PA5-28316

antibody from Invitrogen Antibodies

Targeting: GIT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28316

Provider

Invitrogen Antibodies

Product name

GIT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, Rat2, HUVEC, mouse brain, rat brain.

Concentration

0.65 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GIT1 was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a GIT1 Polyclonal Antibody (Product # PA5-28316) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using GIT1 Polyclonal Antibody (Product # PA5-28316). Sample (30 µg of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. GIT1 Polyclonal Antibody (Product # PA5-28316) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

GIT1 Polyclonal Antibody detects GIT1 protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract.7.5% SDS-PAGE. GIT1 Polyclonal Antibody (Product # PA5-28316) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GIT1 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a GIT1 Polyclonal Antibody (Product # PA5-28316) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GIT1 in methanol-fixed HeLa cells using a GIT1 polyclonal antibody (Product # PA5-28316) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human mixed ovarian cancer, using GIT1 (Product # PA5-28316) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.