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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP32561PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti DJ-1 / PARK7
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic corresponding to amino acids 89-100 of Human DJ-1/PARK7.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot Analysis: Adult human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-PARK7. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PARK7 (~22 kDa).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Proteins were visualized using a Donkey anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PARK7 (~22 kDa).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot Analysis: Brain tissue lysate from human fetus, mouse or rat was resolved by electrophoresis,transferred to PVDF and probed with anti-PARK7. Proteins were visualized using a Donkey anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PARK7 (~22 kDa)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunocytochemistry: Primary murine hippocampal neuronson glial feeder layer, detected by anti-DJ-1/PARK7 (A), anti-MAP2 (B), or merged (C). Images generously provided by Dr. Paul Lockhart, Royal Children's Hospital, Australia.
