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ImmunocytochemistryAntibody data
- Antibody Data
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- References [2]
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- Western blot [2]
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- Product number
- NBP1-92715 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-92715, RRID:AB_11008727
- Product name
- Mouse Monoclonal Park7/DJ-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The Parkinson's Disease-Linked Protein DJ-1 Associates with Cytoplasmic mRNP Granules During Stress and Neurodegeneration.
The positive correlation between DJ-1 and β-catenin expression shows prognostic value for patients with glioma.
Repici M, Hassanjani M, Maddison DC, Garção P, Cimini S, Patel B, Szegö ÉM, Straatman KR, Lilley KS, Borsello T, Outeiro TF, Panman L, Giorgini F
Molecular neurobiology 2019 Jan;56(1):61-77
Molecular neurobiology 2019 Jan;56(1):61-77
The positive correlation between DJ-1 and β-catenin expression shows prognostic value for patients with glioma.
Wang C, Fang M, Zhang M, Li W, Guan H, Sun Y, Xie S, Zhong X
Neuropathology : official journal of the Japanese Society of Neuropathology 2013 Dec;33(6):628-36
Neuropathology : official journal of the Japanese Society of Neuropathology 2013 Dec;33(6):628-36
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Lane view shows a specific band for Park7 (DJ-1) in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Analysis of whole brain and cell lysates using mouse mAb against DJ1/Park7, NBP1-92715, dilution 1:5,000 in green. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The DJ1 antibody detects protein with apparent molecular weight of 21kDa but only in human cell lines, since it does not recognize the rat or mouse DJ1 protein. The blot was simultaneously probed with chicken pAb to vimentin, dilution 1;5,000 in red, revealing a single band at about 50kDa present in all lanes, though at much lower levels in the tissue lysates.
