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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- PA1-37560 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-DJ-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, canine, mouse, porcine and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is normal breast tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 1 mL
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Jurkat Cells using anti-DJ-1 Polyclonal Antibody (Product # PA5-32382). The recommened dilution for this antibody in western blot applications is 1:25.
