NBP2-14786

antibody from Novus Biologicals

Targeting: ATAD2 ANCCA, CT137, DKFZp667N1320, MGC29843, MGC5254, PRO2000

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-14786

Provider

Novus Biologicals

Product name

Mouse Monoclonal ATAD2 Antibody

Antibody type

Monoclonal

Description

Protein A purified. Specificity of human ATAD2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Mouse

Antigen sequence

VGDKRSDPEQ


Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786] - Simple Western lane view shows a specific band for ATAD2 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786] - Electropherogram image(s) of corresponding Simple Western lane view. ATAD2 antibody was used at 1:20 dilution on U-251MG sp lysates(s).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATAD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human rectum shows strong nuclear positivity in a subset of glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining in human testis and skeletal muscle tissues. Corresponding ATAD2 RNA-seq data are presented for the same tissues.