Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- orb137105 - Provider product page
- Provider
- Biorbyt
- Product name
- c-Kit antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody to c-Kit
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 1-3 μg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing
No comments: Submit comment
Supportive validation
- Submitted by
- Biorbyt (provider)
- Main image
- Experimental details
- Western blot analysis of Human Hippocampus lysate (35ug protein in RIPA buffer) using KIT antibody
- Other comments
- Approx 110kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110kDa according to NP_000213.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any such results been reported with other antibodies/lysates. Have any further splice variants/modified forms been reported.
