H00003815-D01P

antibody from Abnova Corporation

Targeting: KIT C-Kit, CD117, PBT, SCFR

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003815-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003815-D01P, RRID:AB_1575618

Product name

KIT purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human KIT protein.

Antigen sequence

MRGARGAWDFLCVLLLLLRVQTGSSQPSVSPGEPS
PPSIHPGKSDLIVRVGDEIRLLCTDPGFVKWTFEI
LDETNENKQNEWITEKAEATNTGKYTCTNKHGLSN
SIYVFVRDPAKLFLVDRSLYGKEDNDTLVRCPLTD
PEVTNYSLKGCQGKPLPKDLRFIPDPKAGIMIKSV
KRAYHRLCLHCSVDQEGKSVLSEKFILKVRPAFKA
VPVVSVSKASYLLREGEEFTVTCTIKDVSSSVYST
WKRENSQTKLQEKYNSWHHGDFNYERQATLTISSA
RVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINI
FPMINTTVFVNDGENVDLIVEYEAFPKPEHQQWIY
MNRTFTDKWEDYPKSENESNIRYVSELHLTRLKGT
EGGTYTFLVSNSDVNAAIAFNVYVNTKPEILTYDR
LVNGMLQCVAAGFPEPTIDWYFCPGTEQRCSASVL
PVDVQTLNSSGPPFGKLVVQSSIDSSAFKHNGTVE
CKAYNDVGKTSAYFNFAFKGNNKEQIHPHTLFTPL
LIGFVIVAGMMCIIVMILTYKYLQKPMYEVQWKVV
EEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTL
GAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAH
LTEREALMSELKVLSYLGNHMNIVNLLGACTIGGP
TLVITEYCCYGDLLNFLRRKRDSFICSKQEDHAEA
ALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTK
ADKRRSVRIGSYIERDVTPAIMEDDELAIDLEDLL
SFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRI
TKICDFGLARDIKNDSNYVVKGNARLPVKWMAHES
IFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPV
DSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDAD
PLKRPTFKQIVQLIEKQISESTNHIYSNLANCSPN
RQKPVVDHSVRINSVGSTASSSQPLLVHDDV


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of KIT expression in transfected 293T cell line (H00003815-T01) by KIT MaxPab polyclonal antibody.Lane 1: KIT transfected lysate(109.90 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between KIT and CSF2RA. HeLa cells were stained with anti-KIT rabbit purified polyclonal 1:1200 and anti-CSF2RA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)