313215

antibody from BioLegend

Targeting: KIT C-Kit, CD117, PBT, SCFR

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

313215

Provider

BioLegend

Proper citation

BioLegend Cat#313215, RRID:AB_10896056

Product name

Brilliant Violet 421™ anti-human CD117 (c-kit) [104D2]

Antibody type

Monoclonal

Antigen

MOLM-1 megakaryocytic cell line

Description

CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemic cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.

Reactivity

Human, Bovine, Simian

Host

Mouse

Conjugate

Blue dye

Isotype

IgG

Vial size

25 tests

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Submitted references

Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood.

Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, Shibuya M, Yoshikawa H, Ohneda O
Blood 2007 Jul 1;110(1):151-60

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human erythroleukemia cell line (HEL) was stained with CD117 (clone 104D2) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).

Conjugate

Blue dye