NBP2-15365

antibody from Novus Biologicals

Targeting: SNCA NACP, PARK1, PARK4, PD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-15365

Provider

Novus Biologicals

Product name

Rabbit Polyclonal alpha-Synuclein Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Neurodegenerative Disease-Related Proteins within the Epidermal Layer of the Human Skin.

Akerman SC, Hossain S, Shobo A, Zhong Y, Jourdain R, Hancock MA, George K, Breton L, Multhaup G
Journal of Alzheimer's disease : JAD 2019;69(2):463-478

---

Apelin-36 mitigates MPTP/MPP⁺-induced neurotoxicity: Involvement of α-synuclein and endoplasmic reticulum stress.

Zhu J, Dou S, Wang C, Jiang Y, Wang C, Cheng B
Brain research 2019 Oct 15;1721:146334

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Synuclein-alpha Antibody [NBP2-15365] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Synuclein-alpha Antibody [NBP2-15365] - Paraffin-embedded Rat brain diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Synuclein-alpha Antibody [NBP2-15365] - Paraffin-embedded rat brain. Green: alpha Synuclein antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen sectioned E13.5 Rat brain. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559 diluted at 1:500. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.