- GTX112799 - GeneTex SNCA antibody

Submitted references MPTP-induced vulnerability of dopamine neurons in A53T α-synuclein overexpressed mice with the potential involvement of DJ-1 downregulation.

Lee S, Oh ST, Jeong HJ, Pak SC, Park HJ, Kim J, Cho HS, Jeon S
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2017 Nov;21(6):625-632

Combined exposure to agriculture pesticides, paraquat and maneb, induces alterations in the N/OFQ-NOPr and PDYN/KOPr systems in rats: Relevance to sporadic Parkinson's disease.

Bastías-Candia S, Di Benedetto M, D'Addario C, Candeletti S, Romualdi P
Environmental toxicology 2015 May-Jun;30(6):656-63

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE GTX112799 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Synuclein antibody detects SNCA protein by western blot analysis.A. 30 ?g SK-N-AS whole cell lysate/extract15% SDS-PAGEalpha Synuclein antibody (GTX112799) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Synuclein antibody detects alpha Synuclein protein by western blot analysis.A. 50 ?g rat brain extract15 % SDS-PAGEalpha Synuclein antibody (GTX112799) dilution: 1:10000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Rat tissue extract (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Mouse tissue extract (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Mouse tissue extract (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Rat tissue extract (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various tissue extracts (50 ?g) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody (GTX112799) diluted at 1:2000 and competitor's antibody (MO22112) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various tissue extracts (50 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX112799) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Synuclein antibody detects SNCA protein at membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: SNCA protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500.Blue: Hoechst 33343 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Synuclein(GTX112799) antibody at 1:250 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in Rat brain by immunohistochemical analysis. Sample: Paraffin-embedded Rat brain. alpha Synuclein antibody (GTX112799) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: alpha Synuclein antibody (GTX112799) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).Blue: Nuclear staining with Hoechst 33342.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX112799) diluted at 1:250.Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

GTX112799

Antibody data