35-8300

antibody from Invitrogen Antibodies

Targeting: SNCA NACP, PARK1, PARK4, PD1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

35-8300

Provider

Invitrogen Antibodies

Product name

alpha Synuclein Monoclonal Antibody (Syn 505)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

Syn 505

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

Human myeloperoxidase (hMPO) is expressed in neurons in the substantia nigra in Parkinson's disease and in the hMPO-α-synuclein-A53T mouse model, correlating with increased nitration and aggregation of α-synuclein and exacerbation of motor impairment.

Maki RA, Holzer M, Motamedchaboki K, Malle E, Masliah E, Marsche G, Reynolds WF
Free radical biology & medicine 2019 Sep;141:115-140

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Chronic mild stress accelerates the progression of Parkinson's disease in A53T α-synuclein transgenic mice.

Wu Q, Yang X, Zhang Y, Zhang L, Feng L
Experimental neurology 2016 Nov;285(Pt A):61-71

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Age-dependent α-synuclein aggregation in the Microcebus murinus lemur primate.

Canron MH, Perret M, Vital A, Bézard E, Dehay B
Scientific reports 2012;2:910

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Novel antibodies to synuclein show abundant striatal pathology in Lewy body diseases.

Duda JE, Giasson BI, Mabon ME, Lee VM, Trojanowski JQ
Annals of neurology 2002 Aug;52(2):205-10

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WT TNM, a recombinant wild-type form of a-Synuclein that has been treated with tetranitromethane, using Ms anti-Nitrated a-Synuclein (Product # 35-8300).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of mouse brain (locus coeruleus) double-labeled with Ms anti-Nitrated a-Synuclein (Product # 35-8300, red) and anti-Tyrosine Hydroxylase (green). Image courtesy of John Trojanowski, MD, PhD, University of Pennsylvania School of Medicine, PA.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Distribution of synuclein and amyloid pathology in mouse lemur brains. (A) Sagittal section of mouse lemur stained with Mayer's hemalum showing midbrain and forebrain regions. Scale bar = 2 mm. (B) Sagittal section of mouse lemur stained with Mayer's hemalun showing distribution of betaA4 (purple stars), alpha-synuclein (pink stars), Phospho-Ser129 alpha-synuclein (orange stars) immunoreactive regions. (C-H) Magnifications of selected alpha-synuclein positive regions in young and old animals boxed in A: cortex (C-D), SNpc (E-F), striatum (G-H). (I-N) Magnifications of selected Phospho-Ser129 alpha-synuclein positive regions in young and old animals boxed in A: cortex (I-J), SNpc (K-L), striatum (M-N). (O-T) Magnifications of selected betaA4 positive regions in young and old animals boxed in A: cortex (O-P), SNpc (Q-R), striatum (S-T); Scale bar = 10 um.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Phosphorylated and nitrated alpha-synuclein immunoreactivity is enhanced in the substantia nigra of old (n = 4) as compared with young (n = 3) mouse lemur. (A) Representative midbrain sections from young and old mouse lemur co-immunostained with tyrosine hydroxylase (TH, red) and an anti-phosphorylated alpha-synuclein (P-Ser129 alpha-synuclein, green). The merged image shows colabeling within a nigral neuron. (B) Double immunofluorescence for nitrated alpha-synuclein (green) in TH-positive neurons (red) in young and old animals. The merged image shows coimmunoreactivity within a nigral neuron. Scale bar for panels A and B = 10 um.