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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- Flow cytometry [1]
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- Product number
- MA5-17123 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP3 Monoclonal Antibody (4F10)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17123 targets MMP3 in FACS, pep-ELISA, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 4F10
- Concentration
- 1 mg/mL
Submitted references Epithelial Migration and Non-adhesive Periderm Are Required for Digit Separation during Mammalian Development.
Interleukin-22 promotes development of malignant lesions in a mouse model of spontaneous breast cancer.
Kashgari G, Meinecke L, Gordon W, Ruiz B, Yang J, Ma AL, Xie Y, Ho H, Plikus MV, Nie Q, Jester JV, Andersen B
Developmental cell 2020 Mar 23;52(6):764-778.e4
Developmental cell 2020 Mar 23;52(6):764-778.e4
Interleukin-22 promotes development of malignant lesions in a mouse model of spontaneous breast cancer.
Katara GK, Kulshrestha A, Schneiderman S, Riehl V, Ibrahim S, Beaman KD
Molecular oncology 2020 Jan;14(1):211-224
Molecular oncology 2020 Jan;14(1):211-224
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP3 using a MMP3 monoclonal antibody (Product # MA5-17123) against a human MMP3 (AA: 189-441) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of MMP3 was achieved by transfecting HeLa cells with MMP3 specific siRNAs (Silencer® select Product # s8854, s8855). Western blot analysis (Fig. a) was performed using whole cell extracts from the MMP3 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed by MMP3 Monoclonal Antibody (Product # MA5-17123) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MMP3.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MMP3 Monoclonal Antibody (4F10), (Product # MA5-17123) and a 53.9 kDa band corresponding to MMP3 was observed in the cell lines. Whole cell extracts (30 µg lysate) of C8D1A (Lane 1), HeLa (Lane 2) and MDA-MB-231 (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP3 using a MMP3 monoclonal antibody (Product # MA5-17123) against a human MMP3 (AA: 189-441) recombinant protein.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using MMP3 monoclonal antibody (Product # MA5-17123) (green) and negative control (red).
