sc-21732

antibody from Santa Cruz Biotechnology

Targeting: MMP3 STMY, STMY1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-21732

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-21732, RRID:AB_627958

Product name

Anti-MMP3

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

References

Submitted references

Matrix metalloproteinase 3 is present in the cell nucleus and is involved in apoptosis.

Si-Tayeb K, Monvoisin A, Mazzocco C, Lepreux S, Decossas M, Cubel G, Taras D, Blanc JF, Robinson DR, Rosenbaum J
The American journal of pathology 2006 Oct;169(4):1390-401

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A reinforced merging methodology for mapping unique peptide motifs in members of protein families.

Chang HT, Pai TW, Fan TC, Su BH, Wu PC, Tang CY, Chang CT, Liu SH, Chang MD
BMC bioinformatics 2006 Jan 25;7:38

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 54

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human prostate shows distinct cytoplasmic positivity in glandular cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.