NBP1-44861

antibody from Novus Biologicals

Targeting: CD81 TAPA-1, TAPA1, TSPAN28

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-44861

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-44861, RRID:AB_10008097

Product name

Mouse Monoclonal CD81 Antibody

Antibody type

Monoclonal

Description

Protein A purified. The antibody M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.

Reactivity

Human, Feline, Rabbit

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Slow Release of HIV-1 Protein Nef from Vesicle-like Structures Is Inhibited by Cytosolic Calcium Elevation in Single Human Microglia.

Stenovec M, Lasič E, Dominkuš PP, Bobnar ST, Zorec R, Lenassi M, Kreft M
Molecular neurobiology 2019 Jan;56(1):102-118

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Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.

Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ
The Journal of biological chemistry 1998 Aug 7;273(32):20121-7

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Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.

Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ
The Journal of biological chemistry 1998 Aug 7;273(32):20121-7

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Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82.

Imai T, Kakizaki M, Nishimura M, Yoshie O
Journal of immunology (Baltimore, Md. : 1950) 1995 Aug 1;155(3):1229-39

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C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells.

Imai T, Yoshie O
Journal of immunology (Baltimore, Md. : 1950) 1993 Dec 1;151(11):6470-81

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Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells.

Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O
Journal of virology 1992 Mar;66(3):1394-401

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Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells.

Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O
Journal of virology 1992 Mar;66(3):1394-401

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Surface staining of CD81 in human peripheral blood with anti-CD81 (M38) APC-CyTM7.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Human peripheral blood with anti-CD81 (M38) APC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Human peripheral blood with anti-CD81 (M38) FITC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Flow Cytometry: CD81 Antibody (M38) [PE] [NBP1-44861PE] - A cell surface stain was performed on THP-1 cells with CD81 antibody (M38) NBP1-44861 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE. Image using the PE form of this antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) PE.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) PE.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) biotin, streptavidin-APC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD81 (M38) purified antibody (concentration in sample 4 ug/ml) GAM APC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Surface staining pattern of human peripheral blood stained using anti-human CD81 (M38) purified antibody (concentration in sample 4 ug/ml) GAM APC.