MAB21501-100

antibody from R&D Systems

Targeting: C5 C5a, C5b, CPAMD4

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB21501-100

Provider

R&D Systems

Product name

Mouse Complement Component C5a Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse Complement Component C5a in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse Complement Component C3d, recombinant human (rh) Complement Component C3a, or rhComplement Component C5a.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

P06684


Isotype

IgG

Antibody clone number

295108

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Real-Time Imaging of Interactions of Neutrophils with Cryptococcus neoformans Demonstrates a Crucial Role of Complement C5a-C5aR Signaling.

Sun D, Zhang M, Liu G, Wu H, Zhu X, Zhou H, Shi M
Infection and immunity 2016 Jan;84(1):216-29

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Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara.

Price PJ, Bánki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, Lehmann MH
Journal of immunology (Baltimore, Md. : 1950) 2015 Feb 1;194(3):1164-8

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

N-Acetyl-beta-D-Glucosaminidase Release Induced by Complement Component C5a and Neutralization by Mouse Complement Component C5a Antibody. Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) induces N-acetyl-beta-D-glucosaminidase release in the the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta-D-Glucosaminidase Release elicited by Recombinant Mouse Complement Component C5a (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Complement Component C5a Monoclonal Antibody (Catalog # MAB21501). The ND₅₀ is typically 0.1-0.5 µg/mL in the presence of cytochalasin-B.