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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [2]
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- Product number
- NB100-56405 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56405, RRID:AB_2186275
- Product name
- Rabbit Polyclonal SENP1 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified. A 66 kDa band is observed. In human and mouse, SENP1 has different isoforms between 664 and 699 amino acids long. Additional bands seen in western blot may represent other SUMOlated proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references SUMO-specific protease 1 protects neurons from apoptotic death during transient brain ischemia/reperfusion.
Developmental control of sumoylation pathway proteins in mouse male germ cells.
Sumoylation of the novel protein hRIP{beta} is involved in replication protein A deposition in PML nuclear bodies.
Zhang H, Wang Y, Zhu A, Huang D, Deng S, Cheng J, Zhu MX, Li Y
Cell death & disease 2016 Nov 24;7(11):e2484
Cell death & disease 2016 Nov 24;7(11):e2484
Developmental control of sumoylation pathway proteins in mouse male germ cells.
La Salle S, Sun F, Zhang XD, Matunis MJ, Handel MA
Developmental biology 2008 Sep 1;321(1):227-37
Developmental biology 2008 Sep 1;321(1):227-37
Sumoylation of the novel protein hRIP{beta} is involved in replication protein A deposition in PML nuclear bodies.
Park J, Seo T, Kim H, Choe J
Molecular and cellular biology 2005 Sep;25(18):8202-14
Molecular and cellular biology 2005 Sep;25(18):8202-14
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SENP1 Antibody [NB100-56405] - Simple Western lane view shows a specific band for SENP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SENP1 Antibody [NB100-56405] - analysis of SENP1 in A) Daudi, B) HeLa and C) NIH 3T3 cell lysate using this antibody.
