AF3296

antibody from R&D Systems

Targeting: ERCC2 EM9, MAG, MGC102762, MGC126218, MGC126219, XPD

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3296

Provider

R&D Systems

Product name

Human/Mouse/Rat XPD Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse, and rat XPD in Western blots.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P18074


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

MAD2 interacts with DNA repair proteins and negatively regulates DNA damage repair.

Fung MK, Han HY, Leung SC, Cheung HW, Cheung AL, Wong YC, Ling MT, Wang X
Journal of molecular biology 2008 Aug 1;381(1):24-34

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat XPD by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat XPD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3296) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPD at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.