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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NB100-41382 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-41382, RRID:AB_2264525
- Product name
- Goat Polyclonal 5-HT3A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise all three reported isoforms (NP_998786.2
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Inactivation of the orphan nuclear receptor TR3/Nur77 inhibits pancreatic cancer cell and tumor growth.
Estrogen rapidly modulates 5-hydroxytrytophan-induced visceral hypersensitivity via GPR30 in rats.
Modulation of dopamine transmission by 5HT2C and 5HT3 receptors: a role in the antidepressant response.
Modulation of dopamine transmission by 5HT2C and 5HT3 receptors: a role in the antidepressant response.
Lee SO, Abdelrahim M, Yoon K, Chintharlapalli S, Papineni S, Kim K, Wang H, Safe S
Cancer research 2010 Sep 1;70(17):6824-36
Cancer research 2010 Sep 1;70(17):6824-36
Estrogen rapidly modulates 5-hydroxytrytophan-induced visceral hypersensitivity via GPR30 in rats.
Lu CL, Hsieh JC, Dun NJ, Oprea TI, Wang PS, Luo JC, Lin HC, Chang FY, Lee SD
Gastroenterology 2009 Sep;137(3):1040-50
Gastroenterology 2009 Sep;137(3):1040-50
Modulation of dopamine transmission by 5HT2C and 5HT3 receptors: a role in the antidepressant response.
Dremencov E, Weizmann Y, Kinor N, Gispan-Herman I, Yadid G
Current drug targets 2006 Feb;7(2):165-75
Current drug targets 2006 Feb;7(2):165-75
Modulation of dopamine transmission by 5HT2C and 5HT3 receptors: a role in the antidepressant response.
Dremencov E, Weizmann Y, Kinor N, Gispan-Herman I, Yadid G
Current drug targets 2006 Feb;7(2):165-75
Current drug targets 2006 Feb;7(2):165-75
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 5-HT3A Antibody [NB100-41382] - Staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: 5-HT3A Antibody [NB100-41382] - Analysis of 5-HT3A in C57BL/6 mouse retina section using anti-5-HT3A antibody. IHC-Fr image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-41382] - Staining of human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: 5-HT3A Antibody [NB100-41382] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
