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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF7688 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Kallikrein 7 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse and rat Kallikrein 7 in Western blots. Detects mouse Kallikrein 7 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Kallikrein 7 is observed, and less than 1% cross-reactivity with recombinant mouse Kallikrein 5 is observed.
- Reactivity
- Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Kallikrein 7 by Western Blot. Western blot shows lysates of mouse skin tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7688) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kallikrein 7 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Rat Kallikrein 7 by Western Blot. Western blot shows lysates of rat skin tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7688) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kallikrein 7 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Kallikrein 7 in Mouse Skin. Kallikrein 7 was detected in perfusion fixed frozen sections of mouse skin using Sheep Anti-Mouse Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7688) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to keratinocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
